Effect of different mechanical surface treatments on flexural strength of repaired denture base / Efecto de diferentes tratamientos superficiales mecánicos sobre la resistencia a la flexión de la base de la dentadura reparada

Aim: To assess the effect of different mechanical surface treatments on flexural strength of repaired denture base. Material and Methods: Sixty bar-shaped specimens of heat-polymerized acrylic resin were fabricated, and divided into six groups (n=10). All specimens, except the positive control group (group PC), were sectioned into halves to create a 1-mm clearance. A negative control group with no surface treatment (group NC) was also considered. Other groups underwent different surface treatments: group Laser; treated with erbium: yttrium-aluminum-garnet (Er:YAG) laser, group APA; airborne-particle abrasion (APA), group APA plus Laser; a combination of laser and APA, and group Bur; bur grinding. After measuring surface roughness (Ra) with a profilometer, all sectioned specimens were repaired by auto-polymerizing acrylic resin, and thermocycled afterward. Three-point bending test was performed by a universal testing machine. Data were statistically analyzed (α=0.05). Results: The mean surface roughness of all experimental groups were significantly higher than that of group NC (p<0.05). The mean flexural strength of all groups was significantly lower than that of group PC (p<0.05). Group B had significantly higher flexural strength than the other surface-treated groups (p<0.05). Group Laser had significantly higher flexural strength than groups APA (p=0.043) and APA plus Laser (p=0.023). No significant difference was found between groups APA and APA plus Laser (p=0.684). Conclusion: All surface treatments increased the surface roughness and flexural strength compared with the untreated group. The highest flexural strength was observed in specimens treated by bur grinding and then laser, however, it was still significantly lower than intact specimens.

Objetivo: Evaluar el efecto de diferentes tratamientos superficiales mecánicos sobre la resistencia a la flexión de la base de la prótesis reparada. Material y Métodos: Se fabricaron sesenta especímenes en forma de barra de resina acrílica termo-polimerizada y se dividieron en seis grupos (n=10). Todas las muestras, excepto el grupo de control positivo (grupo PC), se seccionaron en mitades para crear un espacio libre de 1 mm. También se consideró un grupo de control negativo sin tratamiento superficial (grupo NC). Otros grupos se sometieron a diferentes tratamientos superficiales: grupo Láser; tratados con láser de erbio: itrio-aluminio-granate (Er:YAG), grupo APA; abrasión por partículas en el aire (APA), grupo APA más láser; una combinación de láser y APA, y grupo Bur; molienda de fresas. Después de medir la rugosidad de la superficie (Ra) con un perfilómetro, todas las muestras seccionadas se repararon con resina acrílica de autopolimerización y se sometieron a termociclado. La prueba de flexión de tres puntos se realizó con una máquina de prueba universal. Los datos se analizaron estadísticamente (α=0,05). Resultados: La rugosidad superficial media de todos los grupos experimentales fue significativamente mayor que la del grupo NC (p<0,05). La resistencia media a la flexión de todos los grupos fue significativamente menor que la del grupo PC (p<0,05). El grupo B tenía una resistencia a la flexión significativamente mayor que los otros grupos tratados en la superficie (p<0,05). El grupo Láser tuvo una resistencia a la flexión significativamente mayor que los grupos APA (p=0,043) y APA más Láser (p=0,023). No se encontró diferencia significativa entre los grupos APA y APA más Láser (p=0,684). Conclusión: Todos los tratamientos superficiales aumentan la rugosidad de la superficie y la resistencia a la flexión en comparación con el grupo sin tratar. La resistencia a la flexión más alta se observó en las muestras tratadas con fresado y luego con láser; sin embargo, aún era significativamente más baja que las muestras intactas.

Biceps tenotomy or tenodesis in association with rotator cuff repair: is there an influence on functional results? A retrospective cohort study

ABSTRACT BACKGROUND: Instability or tears of the long head of the biceps tendon (LHBT) may be present in more than 35% of rotator cuff repairs (RCR). OBJECTIVE: To compare clinical results from patients undergoing arthroscopic RCR, according to the procedure performed at the LHBT. DESIGN AND SETTING: Retrospective cohort study designed at the shoulder and elbow clinic of Instituto de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo, Brazil. METHODS: Functional results among patients were compared using the American Shoulder and Elbow Surgeons (ASES) and University of California Los Angeles (UCLA) scales, according to the LHBT approach adopted: no procedure, tenotomy or tenodesis. RESULTS: We evaluated 306 shoulders (289 patients): 133 underwent no procedure at the LHBT, 77 tenotomy and 96 tenodesis. The ASES scale at 24 months showed no difference (P = 0.566) between the groups without LHBT procedure (median 90.0; interquartile range, IQR 29), tenotomy (median 90.0; IQR 32.1) or tenodesis (median 94.4; IQR 22.7); nor did the UCLA scale (median 33; IQR 7 versus median 31; IQR 8 versus median 33; IQR 5, respectively, P = 0.054). The groups differed in the preoperative functional assessment according to the ASES and UCLA scale, such that the tenodesis group started from higher values. However, there was no difference in pre and postoperative scores between the groups. CONCLUSION: Tenodesis or tenotomy of the LHBT, in the sample analyzed, did not influence the clinical results from RCR, as assessed using the ASES and UCLA scales.

Definiciones de los reparos, marcas óseas o accidentes de la superficie del hueso en anatomía humana / Definitions of the landmarks, bony marks or accidentes of the bone surface in human anatomy

RESUMEN: El aprendizaje de la Anatomía puede resultar un proceso dificultoso y poco motivador para el estudiante, esto debido a su complejo vocabulario. Uno de los desafíos iniciales en el proceso de enseñanza-aprendizaje de la Anatomía Humana es el abordaje de los términos generales del sistema musculoesquelético agrupados con los nombres de reparos, marcas óseas o accidentes de la superficie del hueso. Estos suelen ser mencionados con una escasa o nula definición en los recursos bibliográficos. El objetivo de este trabajo fue revisar los recursos bibliográficos que efectivamente cuentan con definiciones de los términos denominados como reparos, marcas óseas o accidentes de la superficie del hueso, con la finalidad de aportan información aclaratoria sobre el uso de estos términos óseos y así facilitar los procesos de enseñanza-aprendizaje de la Anatomía Humana. Los términos utilizados comúnmente para describir reparos, marcas óseas o accidentes de la superficie del hueso fueron analizados en Terminologia Anatomica (FIPAT, 2019) y en cinco textos de Anatomía Humana. Para facilitar la comprensión, los resultados fueron clasificados y tabulados Se pudo evidenciar una escasez de recursos bibliográficos con definiciones de los reparos, marcas óseas o accidentes de la superficie del hueso, la mayoría de estos términos óseos se encontraron en el sistema musculoesquélico, sin embargo, no son exclusivos de este sistema, además, se observaron errores conceptuales en las definiciones de algunos de los términos óseos. Una manera de comprender mejor estos términos, es revisar de manera frecuente la Terminologia Anatomica.

SUMMARY: Learning Anatomy can be a difficult and unmotivating process for the student, due to its complex vocabulary. One of the initial challenges in the teaching-learning process of Human Anatomy is the approach to the general terms of the musculoskeletal system grouped with the names of repairs, bone marks or accidents on the surface of the bone. These are usually mentioned with little or no definition in bibliographic resources. The objective of this work was to review the bibliographic resources that effectively have definitions of the terms known as repairs, bone marks or accidents on the bone surface, in order to provide clarifying information on the use of these bone terms and thus facilitate the teaching-learning processes of Human Anatomy. The terms commonly used to describe repairs, bone marks or accidents on the bone surface were analyzed in Terminologia Anatomica (FIPAT, 2019) and in five texts on Human Anatomy. To facilitate understanding, the results were classified and tabulated. There was a lack of bibliographic resources with definitions of the repairs, bone marks or accidents on the bone surface. Most of these bone terms were found in the musculoskeletal system. However, they are not exclusive to this system, and conceptual errors were observed in the definitions of some of the bone terms. One way to better understand these terms is to frequently review the Terminologia Anatomica.

Surface Treatments for Repair of Feldspathic, Leucite - and Lithium Disilicate-Reinforced Glass Ceramics Using Composite Resin

The aim of this study was to evaluate the efficacy of different surface conditioning methods on the microtensile bond strength of a restorative composite repair in three types of dental ceramics: lithium disilicate-reinforced, leucite-reinforced and feldspathic. Twelve blocks were sintered for each type of ceramic (n=3) and stored for 3 months in distilled water at 37 °C. The bonding surface of ceramics was abraded with 600-grit SiC paper. Surface treatments for each ceramic were: GC (control) - none; GDB - diamond bur #30 µm; GHF - hydrofluoric acid (10%); GT- tribochemical silica coating (45-μm size particles). Treatments were followed by cleaning with phosphoric acid 37% for 20 s + silane + adhesive. The composite resin was used as restorative material. After repair, samples were subjected to thermocycled ageing (10,000 cycles between 5 °C and 55 °C for 30 s). Thereafter, the samples were sectioned into 1.0 mm2 sticks and tested for microtensile bond strength with 0.5 mm/min crosshead speed. Data were compared by two-way ANOVA and Tukey's test (α=0.05). The superficial wear with diamond bur proved to be suitable for feldspathic porcelain and for leucite-reinforced glass ceramic while hydrofluoric acid-etching is indicated for repairs in lithium disilicate-reinforced ceramic; tribochemical silica coating is applicable to leucite-reinforced ceramic. Predominance of adhesive failures was observed (>85% in all groups). In conclusion, the success of surface treatments depends on the type of ceramic to be repaired.

O objetivo deste estudo foi avaliar a eficácia de diferentes condicionamentos de superfície na resistência de união de reparos de compósitos restauradores em três tipos de cerâmicas odontológicas: reforçada por dissilicato de lítio, reforçada por leucita e feldspática. Foram confeccionados 12 blocos para cada tipo de cerâmica (n=3) e armazenados por 3 meses em água destilada a 37 oC. A superfície de união das cerâmicas foi regularizada com lixa de granulação 600 por 15 s e lavadas em ultrassom por 10 min. Os tratamentos de superfície para cada cerâmica foram: GC (controle) - nenhum; GPD - ponta diamantada com 30 µm de granulação; GAF - ácido hidrofluorídrico a 10%; GJ - jateamento com partículas de óxido de alumínio revestido por sílica (45 µm - tamanho das partículas). Após, foi realizada a limpeza da superfície com ácido fosfórico a 7% por 20 s, seguido de silano e adesivo. Como material restaurador foi utilizada resina composta. Após o reparo, as amostras foram submetidas a ciclagem térmica (10,000 ciclos entre 5 °C e 55 °C, por 30 s). Na sequência, as amostras foram seccionadas em palitos de aproximadamente 1,0 mm2 e levadas ao teste de tração em uma máquina de ensaios universal à velocidade de 0,5 mm/min. Os dados obtidos foram comparados estatisticamente por ANOVA de dois fatores e teste de Tukey (α=0,05). Sugere-se que o desgaste da superfície com ponta diamantada é mais indicado para a cerâmica feldspática e cerâmica reforçada por leucita, enquanto o condicionamento com ácido fluorídrico é indicado para reparos em cerâmica reforçada por dissilicato de lítio. O jateamento com partículas de óxido de alumínio revestido por sílica mostrou-se aplicável à cerâmica reforçada por leucita. Predominancia de fraturas adesivas acima de 85% foi observada para todos os grupos. Este estudo demonstrou que o sucesso dos tratamentos de superfície depende do tipo de cerâmica a que são aplicados.

A membrane controlled release drug delivery system promotes injured tissue repair / 中国组织工程研究

10.3969/j.issn.2095-4344.2013.25.019

Construction of Eukaryotic Expression Plasmid of bFGF Gene in Rats and Its Expression in Tenocytes / 华中科技大学学报（医学）（英德文版）

The bFGF plays an important role in embryonic development of tendons and ligaments and in the healing of injuried tendons and ligaments. The eukaryotic expression plasmid of rat basic fibroblast growth factor (bFGF) gene was constructed in order to further investigate the bFGF function in molecular regulatory mechanism in the repair of tendons and ligaments and to provide the foundation for the clinical application. The cDNA fragments of bFGF were cloned from the skin of rats by RT-PCR, and recombinated to the pMD18-T vector. The cDNA encoding bFGF was cloned from the pMD18-T vector by RT-PCR, digested with restriction enzyme EcoR Ⅰ, Pst Ⅰ and bound to eukaryotic expression plasmid pIRES2-EGFP to construct eukaryotic expression plasmid pIRES2-EGFP-bFGF. The pIRES2-EGFP-bFGF was transfected into the tenocytes by lipid-mediated ransfection technique. MTT test was used to detect the biological activity of bFGF in supematants after the transfection. The expression of type Ⅰ and Ⅲ collagen genes was detected by using RT-PCR. It was verified that the pIRES2-EGFP-bFGF was successfully constructed, and its transfection into tenocytes could significantly enhance the biological activity of bFGF, and increase the expression of type Ⅰ and Ⅲ collagen mRNA, suggesting that pIRES2-EGFP-mediated bFGF gene therapy was beneficial to the repair of tendons and ligaments.

Comparison of One and Two Stage Repairs in 83 Cases of Hypospadias / 대한비뇨기과학회지

The majority of hypospadias repairs can now be accomplished in one stage, providing a better result with reduced morbidity. To evaluate the efficacy of one stage repairs of hypospadias, a total of 83 patients treated with various urethroplasty technique was stratified according to 2 different stage repairs of hypospadias and compared to clinical outcome during the period of the last 15 years. The study included the age of the patients, anatomical variants of the hypospadias, accompanying congenital anomaly, the technique used, postoperative complication and management, success rate according to the technique used and the methods of urinary diversion. The method of repair was based on individual patient pathology. Transverse preputial island flap was the most common operation performed (n=24) followed by parameatal-based flap (n=15), meatal advancement and granuloplasty repair (MAGPI) in one stage repairs. Thiersch-Duplay (n=8), Belt-Fuqua (n=3), Byars (n=3), Modified Denis-Brown (n=7) techniques were performed in two stage repairs. Of the 83 cases of hypospadias, one stage repairs were done in 62 cases and two stage repairs in 21 cases and their successful outcome, defined as a controllable urinary stream, an acceptable cosmetic appearance and functional erection, was achieved in 60% and 51% respectively. The postoperative complication were fistula in 25 cases (18 in one stage repairs/7 in two stage repairs), stricture in 6 (4/2), wound dehiscence in 2 (1/1), diverticulum in 1 (1/0), persistent chordee in 1 (1/0). There were no significant difference between one and two stage repairs in success rate (60%/ 51%), so we thought that one stage hypospadias repairs could be applicable in most cases of hypospadias and gave satisfactory cosmetic and functional results with a low incidence of serious complications and low costs.

Research on Combat Resilience of Medical Equipment in Battlefield / 医疗卫生装备

Objective To study the combat resilience and battlefield damage probability model for field medical equipment in order to make a valuable reference to the decision-making of medical organization for the further medical support.Methods According to the characteristics of combat resilience of medical equipment in battlefield,the probability of the combat resilience and mean time of recovery repair were analyzed.From making a scientific assumption,the battlefield damage probability model of medical equipment was deduced.Results Based on the systemic analysis,the countermeasures to shorten the time of rush repairs and increase the effect of rush repairs were made.Conclusion Battlefield damage analysis process provides systematic idea and method for medical equipment damage analysis.It is a practical method for achieving assessment damage information and a theoretical basis to dispose the maintenance resources in battlefield.